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wa09 lt2e h9caggfp p80 xx  (WiCell Research Institute Inc)


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    WiCell Research Institute Inc wa09 lt2e h9caggfp p80 xx
    Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: <t>WA09,</t> HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .
    Wa09 Lt2e H9caggfp P80 Xx, supplied by WiCell Research Institute Inc, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Sex-biased gene expression during neural differentiation of human embryonic stem cells"

    Article Title: Sex-biased gene expression during neural differentiation of human embryonic stem cells

    Journal: Frontiers in Cell and Developmental Biology

    doi: 10.3389/fcell.2024.1341373

    Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: WA09, HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .
    Figure Legend Snippet: Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: WA09, HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .

    Techniques Used: RNA Sequencing Assay, Expressing, Marker

    hESC lines and gene expression analysis method and timepoints.
    Figure Legend Snippet: hESC lines and gene expression analysis method and timepoints.

    Techniques Used: Expressing



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    Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: <t>WA09,</t> HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .
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    Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: <t>WA09,</t> HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .
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    Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: WA09, HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Sex-biased gene expression during neural differentiation of human embryonic stem cells

    doi: 10.3389/fcell.2024.1341373

    Figure Lengend Snippet: Similarities between male and female hESC in neuronal differentiation: (A) Overview of the experimental procedure: Four hESC lines of each sex were differentiated for 37 days to a population of mixed neurons induced by small molecules in 2D culture. Bulk RNA sequencing and qPCR. (B) A PCA analysis of expressed genes displays similar gene expression patterns at D0, some inter-cell line differences during early differentiation, and a sex-biased separation at D37 (XY: WA14, HS401, HS983a, XX: WA09, HS975, HS980). (C) Marker gene expression for pluripotency (OCT4, NANOG), ectoderm (PAX6, SOX1), immature neurons (DCX, NCAM), mature neurons (MAP2, DLG4, SYP), post-mitotic neurons (ELAVL2, TUBA1A, NDN), as well as for proliferation (MKI67, PCNA), and apoptosis (CASP3, BCL2) show a similar expression in male and female cell lines. (D) Markers for specialized neurons (GABAergic, Dopaminergic, Glutamatergic) display a premature state of the neurons after 37 days of differentiation. (E) A gene set enrichment analysis at D4, D9, and D37 reveals an upregulation of biological processes implicated in neuronal differentiation in all cell lines. The GSEA was summarized in an enrichment map. Each dot represents a gene set; the size of the dot corresponds to the size of the gene set. If the dots are connected or in close proximity, the more the gene sets are overlapping, and the closer related are their associated biological functions. Gene sets were grouped by a common GO-term classification and named after an overarching biological function. Biological functions implicated in neuronal development are highlighted in blue. Gene sets are filtered by FDR q-values <0.1. created with BioRender.com .

    Article Snippet: The human embryonic stem cell lines WA14 p33 (XY) ( ) and WA09 LT2e-H9CAGGFP p80 (XX) ( ) were acquired from WiCell, Madison, Wisconsin.

    Techniques: RNA Sequencing Assay, Expressing, Marker

    hESC lines and gene expression analysis method and timepoints.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Sex-biased gene expression during neural differentiation of human embryonic stem cells

    doi: 10.3389/fcell.2024.1341373

    Figure Lengend Snippet: hESC lines and gene expression analysis method and timepoints.

    Article Snippet: The human embryonic stem cell lines WA14 p33 (XY) ( ) and WA09 LT2e-H9CAGGFP p80 (XX) ( ) were acquired from WiCell, Madison, Wisconsin.

    Techniques: Expressing